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Sample GSM1630153 Query DataSets for GSM1630153
Status Public on Mar 11, 2015
Title reproductively active turkey rep 4
Sample type RNA
 
Source name Pituitary gland of reproductively active turkey
Organism Meleagris gallopavo
Characteristics gender: Female
developmental stage: Adult
reproductive stage: Laying
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) following manufacturer’s instructions
Label Alexa fluor 555, Alexa fluor 647
Label protocol cDNA was synthesized and fluorescently labelled using SuperScript Plus Indirect cDNA Labeling System (Invitrogen) following manufacturer’s instructions
 
Hybridization protocol Hybridisation was performed at 42°C for 16 hr using SlideHyb Buffer (Applied Biosystems) in hybridization chambers. The arrays were washed with increasing stringency using salt sodium citrate and immediately scanned
Scan protocol The arrays were scanned on a ScanArray 5000 (Packard BioScience)
Data processing The fluorescence intensity of each spot from the images was extracted using BlueFuse software (BlueGnome Ltd.) and exported to JMP Genomics (SAS Institute Inc.). The raw intensity was first transformed to log-base 2 and performed within-array Loess normalization and then standardized to a mean of 0 and standard deviation of 1 across arrays.
 
Submission date Mar 09, 2015
Last update date Mar 11, 2015
Contact name Sunantha Kosonsiriluk
E-mail(s) koson003@umn.edu
Organization name University of Minnesota
Department Animal Science
Lab El Halawani
Street address 495 AnSci/VetMed 1988 Fitch Ave
City Saint Paul
State/province Minnesota
ZIP/Postal code 55108
Country USA
 
Platform ID GPL19870
Series (1)
GSE66704 Validation of chicken array for gene expression analysis in turkey

Data table header descriptions
ID_REF
VALUE Mean loess normalized log2 signal intensity

Data table
ID_REF VALUE
A01 0.442211464
A02 0.092640276
A03 -0.050000208
A04 0.263467439
A05 0.350709145
A06 2.335311561
A07 1.474462803
A08 -1.277812816
A09 0.630932473
A10 -0.27005875
A11 3.260131196
A12 2.478442228
A13 0.908445791
A14 0.990443215
A15 0.856235135
A16 1.768671755
A17 -0.735692405
A18 2.19985081
RIGG00001 1.568637531
RIGG00002 -0.662434242

Total number of rows: 20478

Table truncated, full table size 446 Kbytes.




Supplementary file Size Download File type/resource
GSM1630153_ch1_rat4_ch2_rit4.xls.gz 1.9 Mb (ftp)(http) XLS
GSM1630153_ch1_rit4_ch2_rat4.xls.gz 1.9 Mb (ftp)(http) XLS
Processed data included within Sample table

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