|
| Status |
Public on Mar 13, 2015 |
| Title |
cultured satellite cells PRMT5 KO Rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
cultured satellite cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: Pax7CreERT2/Prmt5loxP/loxP (+TAM)
tissue: limb and trunk muscles
cell type: satellite cells
age: adult
genotype: PRMT5 KO
background strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy micro Kit (Qiagen); DNase-Free DNase Set (Qiagen);RiboMinus Eukaryote System v2 (Life Technologies);
Ion Total RNA-Seq Kit v2 (Life Technologies)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
trimming with Reaper version 13-100: -clean-length 40 -qqq-check 53/10 -trim-length 150
mapping to mm10 by STAR 2.4.0a: --outFilterMismatchNoverLmax 0.1 --chimSegmentMin 18 --chimScoreMin 12
differential expression by DESeq2 version 1.62
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: tab-delimited text file produced by DESeq2 bearing differential expression analyses results
|
| |
|
| Submission date |
Mar 12, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mario Looso |
| E-mail(s) |
mario.looso@mpi-bn.mpg.de
|
| Organization name |
Max Planck Institute for Heart and Lung Research
|
| Street address |
Ludwigstrasse 43
|
| City |
Bad Nauheim |
| ZIP/Postal code |
61231 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18635 |
| Series (1) |
| GSE66822 |
Prmt5 is a crucial regulator of muscle stem cell expansion in adult mice |
|
| Relations |
| BioSample |
SAMN03401907 |
| SRA |
SRX954687 |
