|
| Status |
Public on Mar 13, 2015 |
| Title |
U937_EVI1 DMSO replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
U937_EVI1 cells, 24 h DMSO treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U937
protocol: EVI1 expression vector
agent: DMSO
|
| Treatment protocol |
Logarithmically growing cells were treated with 1µM all-trans retinoic acid (ATRA) or an equivalent amount of solvent (DMSO) for 24 h
|
| Growth protocol |
RPMI 10% FCS 1% Penicillin-Streptomycin-Glutamin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
The Affymetrix GeneChip 3’ IVT Express Protocol was used for the preparation of biotinylated amplified RNA (aRNA) from 250ng of total RNA.
|
| |
|
| Hybridization protocol |
12.5 µg of fragmented and labeled aRNA were hybridized to Affymetrix Human Affymetrix GeneChip Human Genome U133 plus 2.0 arrays for 16 h at 45 °C in a GeneChip hybridization oven 640.
|
| Scan protocol |
GeneChips were washed and stained in an Affymetrix Fluidics Station FS450, and the fluorescent signals were measured with an Affymetrix GeneChip Scanner 3000 7G. Fluidics and scan functions were controlled the by the Affymetrix GeneChip Command Console software v3.2 (AGCC).
|
| Data processing |
Gene level normalized expression signals were calculated from Affymetrix CEL files using the RMA algorithm (Irizarry et al., 2003) in the Affymetrix GeneChip Expression Console v1.3 software.
|
| |
|
| Submission date |
Mar 13, 2015 |
| Last update date |
Mar 13, 2015 |
| Contact name |
Rotraud Wieser |
| E-mail(s) |
rotraud.wieser@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Clinic of Medicine I
|
| Street address |
Waehringer Guertel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE66854 |
The oncogene EVI1 enhances transcriptional and biological responses of human myeloid cells to all-trans retinoic acid [U937] |
|
