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Sample GSM1637

Query DataSets for GSM1637

Status

Public on May 01, 2002

Title

BYA-BY

Sample type

RNA

Channel 1

Source name

independent culture of BY4716

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from the lab strain BY4716 (isogenic to S288c).
See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

rbrem@fhcrc.org

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE38

BY_wild_parents

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1424	69	2238	159	0.251412	0
1	829	68	1192	152	0.084431	0
2	827	69	985	151	-0.228335	0
3	890	73	984	154	-0.343409	0
4	405	79	582	160	0.006186	0
5	1239	82	1525	159	-0.126616	0
6	675	72	834	149	-0.182239	0
7	1257	73	1914	149	0.209814	0
8	947	72	1688	147	0.450327	0
9	613	71	975	146	0.246895	0
10	1029	73	1720	152	0.347658	0
11	7125	82	7862	161	-0.237329	0
12	7610	87	4969	163	-1.012656	0
13	2276	82	2965	158	-0.010719	0
14	778	81	957	162	-0.176388	0
15	4001	99	4716	178	-0.148342	0
16	4579	101	5657	180	-0.075653	0
17	2888	84	4215	163	0.164963	0
18	1633	90	2368	178	0.139008	0
19	10475	98	15742	187	0.217804	0

Total number of rows: 7085

Table truncated, full table size 216 Kbytes.

Supplementary data files not provided