|
| Status |
Public on Aug 02, 2016 |
| Title |
Hh activation_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
colon of a mouse with Hh activation
|
| Organism |
Mus musculus |
| Characteristics |
tissue: colon
Sex: male
genotype: Col1a2CreER; Ptch1fl/fl
|
| Treatment protocol |
Tamoxifen was diluted at 20mg/ml in corn oil, sonicated at 37°C for 30 min and injected at 5 mg/25 g mouse i.p. One week later, mice were sacrificed, the colon extracted and a 1 cm piece starting 1.5 cm from the anus immediately frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy mini extraction kits were used according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
affymetrix standard protocols were used for labeling
|
| |
|
| Hybridization protocol |
affymetrix standard protocols were used for hybridization
|
| Scan protocol |
affymetrix standard protocols were used for scanning
|
| Description |
expression data after Hh activation
|
| Data processing |
Affymetrix Eexpression Console was used for analysis with following settings: med-polish (rma-bg,quant-norm, sketch=-1, bioc=true, lowprecision=false, usepm=true, target=0, doavg=false)
|
| |
|
| Submission date |
Mar 23, 2015 |
| Last update date |
Aug 03, 2016 |
| Contact name |
Rune Toftgård |
| E-mail(s) |
rune.toftgard@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Biosciences and Nutrition
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| ZIP/Postal code |
14183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE67172 |
Gene expression in murine colon mucosa following activation of stromal Hedgehog signalling |
|
