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Sample GSM1645 Query DataSets for GSM1645
Status Public on May 01, 2002
Title RMA-BY
Sample type RNA
 
Channel 1
Source name independent culture of wild strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from the lab strain BY4716 (isogenic to S288c)
and a wild isolate strain from a California vineyard
collected by R. Mortimer. See Brem et al., Science
296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE38 BY_wild_parents

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1160 93 1375 130 0.342663 0
1 499 87 795 121 0.830182 0
2 378 79 626 112 0.901700 0
3 798 79 654 109 -0.279658 0
4 275 79 361 113 0.459564 0
5 1006 92 951 122 -0.020745 0
6 755 76 740 108 0.016591 0
7 943 79 1014 107 0.190149 0
8 721 78 927 104 0.476151 0
9 402 77 543 100 0.566945 0
10 715 89 885 120 0.409375 0
11 3677 100 5151 128 0.609877 0
12 6343 107 2739 123 -1.133181 0
13 1590 99 1674 116 0.183493 0
14 457 85 552 108 0.375335 0
15 2430 92 2555 113 0.182866 0
16 2688 93 2865 110 0.206395 0
17 1511 88 1766 109 0.339716 0
18 942 91 1162 122 0.409430 0
19 2629 93 13401 127 2.508054 0

Total number of rows: 7085

Table truncated, full table size 213 Kbytes.




Supplementary data files not provided
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