|
| Status |
Public on Apr 01, 2017 |
| Title |
genomic DNA from blood sample 3 |
| Sample type |
genomic |
| |
|
| Source name |
blood sample 3
|
| Organism |
Homo sapiens |
| Characteristics |
male: 1
age: 42
black: 0
ards: 1
tissue: whole blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
For each selected sample, 800ng DNA was submitted for bisulfite conversion (EZ-96 DNA Methylation Kit, Zymo Research). Approximately 150 ng of bisulfite-converted DNA from each sample was used for profiling DNA methylation levels using the Illumina Infinium HumanMethylation 450 BeadChip (450K array)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
KS003
whole blood DNA samples from existing Chicago, IL cohorts
|
| Data processing |
the M-values (log2 ratio of the intensities of modified probe versus unmodified probe) (30) of CpG probes that passed quality control based on detection p-value across at least 95% of samples were summarized and batch corrected using COMBAT
|
| |
|
| Submission date |
Apr 02, 2015 |
| Last update date |
Apr 01, 2017 |
| Contact name |
Wei Zhang |
| E-mail(s) |
wei.zhang1@northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
680 N. Lake Shore Drive, Suite 1400
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE67530 |
Epigenetic Contribution of the Myosin Light Chain Kinase Gene to Acute Respiratory Distress Syndrome |
|
