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Sample GSM1648899 Query DataSets for GSM1648899
Status Public on Apr 01, 2017
Title genomic DNA from blood sample 4
Sample type genomic
 
Source name blood sample 4
Organism Homo sapiens
Characteristics male: 0
age: 78
black: 0
ards: 0
tissue: whole blood
Extracted molecule genomic DNA
Extraction protocol For each selected sample, 800ng DNA was submitted for bisulfite conversion (EZ-96 DNA Methylation Kit, Zymo Research). Approximately 150 ng of bisulfite-converted DNA from each sample was used for profiling DNA methylation levels using the Illumina Infinium HumanMethylation 450 BeadChip (450K array)
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
 
Hybridization protocol bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description KS004
whole blood DNA samples from existing Chicago, IL cohorts
Data processing the M-values (log2 ratio of the intensities of modified probe versus unmodified probe) (30) of CpG probes that passed quality control based on detection p-value across at least 95% of samples were summarized and batch corrected using COMBAT
 
Submission date Apr 02, 2015
Last update date Apr 01, 2017
Contact name Wei Zhang
E-mail(s) wei.zhang1@northwestern.edu
Organization name Northwestern University
Street address 680 N. Lake Shore Drive, Suite 1400
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL13534
Series (1)
GSE67530 Epigenetic Contribution of the Myosin Light Chain Kinase Gene to Acute Respiratory Distress Syndrome

Data table header descriptions
ID_REF
VALUE Combat_Corrected_AvgBeta

Data table
ID_REF VALUE
cg00000029 0.513912014678505
cg00000108 0.94671102561986
cg00000109 0.865831736587447
cg00000165 0.182485055727454
cg00000236 0.680112313206771
cg00000289 0.664467455833853
cg00000292 0.793120220675963
cg00000321 0.27257810346186
cg00000363 0.266604163003352
cg00000622 0.00422525140904297
cg00000658 0.764349917031848
cg00000714 0.137020075724798
cg00000721 0.933483172876973
cg00000734 0.0746810633082548
cg00000769 0.0343755657987743
cg00000807 0.823950885560125
cg00000884 0.853213374135882
cg00000905 0.0775000800158106
cg00000924 0.504117237539461
cg00000948 0.85047928195157

Total number of rows: 485577

Table truncated, full table size 13865 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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