|
Status |
Public on Jan 14, 2016 |
Title |
O, 80 Hx |
Sample type |
SRA |
|
|
Source name |
Bone marrow mesenchymal stem cell
|
Organism |
Homo sapiens |
Characteristics |
cell type: Bone marrow mesenchymal stem cell donors age group: Old culture condition: Hypoxic paasage: 15
|
Growth protocol |
MSC were expended under normal oxygen condititon vs hypoxia (5%) oxygen condition
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from MSC cells were extracted using miRNA easy RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
ncRNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
micro RNA
|
Data processing |
CLC genomic workbench 7.0 Illumina adapter sequences and sequences tags of length less than 17 and more than 27 were removed miRNA sequences were aligned to human genome (homo sapiens GRCh 37.57) and known miRNA database (miRBase-19) Statistical analysis: Bonferroni and FDR corrected Filtering: Significant, above 2; Kalz test: FDR pvalue <0.05; Kalz test: FC abs value>2 Genome_build: miRBase 19 Supplementary_files_format_and_content: Excel files xlsx containing expression values of Hx normalized against nx MSC
|
|
|
Submission date |
Apr 07, 2015 |
Last update date |
May 15, 2019 |
Contact name |
norlaily mohd ali |
E-mail(s) |
norlailyma@gmail.com
|
Organization name |
Universiti Tunku Abdul Rahman
|
Department |
Faculty of Medicine and Health Sciences
|
Street address |
Universiti Tunku Abdul Rahman, Jln Sg Long
|
City |
Cheras |
State/province |
Selangor |
ZIP/Postal code |
43000 |
Country |
Malaysia |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE67630 |
Impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells |
|
Relations |
BioSample |
SAMN03463652 |
SRA |
SRX981080 |