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Sample GSM1652419 Query DataSets for GSM1652419
Status Public on Jan 14, 2016
Title O, 80 Hx
Sample type SRA
 
Source name Bone marrow mesenchymal stem cell
Organism Homo sapiens
Characteristics cell type: Bone marrow mesenchymal stem cell
donors age group: Old
culture condition: Hypoxic
paasage: 15
Growth protocol MSC were expended under normal oxygen condititon vs hypoxia (5%) oxygen condition
Extracted molecule total RNA
Extraction protocol RNA from MSC cells were extracted using miRNA easy
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy ncRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina HiSeq 2000
 
Description micro RNA
Data processing CLC genomic workbench 7.0
Illumina adapter sequences and sequences tags of length less than 17 and more than 27 were removed
miRNA sequences were aligned to human genome (homo sapiens GRCh 37.57) and known miRNA database (miRBase-19)
Statistical analysis: Bonferroni and FDR corrected
Filtering: Significant, above 2; Kalz test: FDR pvalue <0.05; Kalz test: FC abs value>2
Genome_build: miRBase 19
Supplementary_files_format_and_content: Excel files xlsx containing expression values of Hx normalized against nx MSC
 
Submission date Apr 07, 2015
Last update date May 15, 2019
Contact name norlaily mohd ali
E-mail(s) norlailyma@gmail.com
Organization name Universiti Tunku Abdul Rahman
Department Faculty of Medicine and Health Sciences
Street address Universiti Tunku Abdul Rahman, Jln Sg Long
City Cheras
State/province Selangor
ZIP/Postal code 43000
Country Malaysia
 
Platform ID GPL11154
Series (1)
GSE67630 Impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells
Relations
BioSample SAMN03463652
SRA SRX981080

Supplementary file Size Download File type/resource
GSM1652419_80_Hx_80Hx_sample_grouped_on_mature.xlsx 62.9 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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