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Sample GSM1652582 Query DataSets for GSM1652582
Status Public on Jul 28, 2022
Title control cerebellar tissue, biological rep4
Sample type RNA
 
Source name control cerebellar tissue
Organism Mus musculus
Characteristics strain: C57BL6
tissue: cerebellar tissue
age: Embryonic day 13.5
genotype: control
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using RNeasy® Mini Kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix small sample amplification protocol from app. 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from control cerebellar tissue
Data processing Affymetrix raw data was normalized using the PLIER algorithms (Affymetrix Technical Note. Guide to Probe Logarithmic Intensity Error Estimation. http://affymetrix.com/support/technical/technotesmain.affx). The data from the three comparison groups were from three batches of Affymetrix experiments, and were normalized separately.
 
Submission date Apr 07, 2015
Last update date Jul 28, 2022
Contact name L. H.
Organization name Uppsala University
Street address Dag Hammarskjölds Väg 20
City Uppsala
ZIP/Postal code 751 85
Country Sweden
 
Platform ID GPL6246
Series (1)
GSE77049 Cerebellar gene expression changes in Pdgfc and Pdgfra mutant

Data table header descriptions
ID_REF
VALUE PLIER processed signal intensity

Data table
ID_REF VALUE
10338001 4568.92
10338002 269.31
10338003 1613.08
10338004 991.04
10338005 2.84
10338006 6.23
10338007 14.77
10338008 27.59
10338009 630.96
10338010 3.41
10338011 130.34
10338012 4.07
10338013 1.89
10338014 1.85
10338015 2.08
10338016 478.61
10338017 9572.91
10338018 281.53
10338019 90.89
10338020 440.09

Total number of rows: 35557

Table truncated, full table size 537 Kbytes.




Supplementary file Size Download File type/resource
GSM1652582_J.A.5.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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