|
| Status |
Public on Sep 25, 2019 |
| Title |
Smoke_fetal_lung_rep2 [mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
Fetal lung, E18.5, from mother exposed to smoke during pregnancy
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: Balb/c
genotype/variation: WT
tissue: fetal lung
age: E18.5
gender: male
treatment: mother exposed to smoke during pregnancy
|
| Treatment protocol |
Mice were treated as described in the paper. Briefly, eight-week-old virgin females were mated with each one proven male breeder for 24h. To allow proper implantation of the fertilized oocyte, mice were exposed from gestational day (GD) 2.5 to smoke or filtered air until GD 17.5.
|
| Growth protocol |
Animals housed under spf conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Ovation PicoSL WTA System V2 and Encore Biotin Module (Nugen).
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit).
|
| Scan protocol |
According to the Affymetrix expression protocol with GeneChip Scanner 3000 7G.
|
| Description |
Smoke_M191
Gene expression data from fetal lung, E18.5, from mother exposed to smoke during pregnancy.
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.3.0.187) including median polish and Sketch-Quantile normalisation.
|
| |
|
| Submission date |
Apr 14, 2015 |
| Last update date |
Sep 26, 2019 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE67883 |
Deregulated pulmonary growth hormones after intrauterine smoke exposure |
| GSE67888 |
Deregulated pulmonary growth hormones after intrauterine smoke exposure |
|
