|
| Status |
Public on Apr 25, 2015 |
| Title |
SkeletalMuscle_PD [EP17] |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis_prediabetics
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: pre-diabetics
gender: male
age: 56y
tissue: skeletal muscle (vastus lateralis)
|
| Treatment protocol |
Human: Healthy vs Pre-diabetics vs Type 2 diabetics;
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (including microRNAs) was extracted from frozen tissues using the TRIZOL reagent (Invitrogen) following the manufacturer’s recommended protocol. Concentration and purity of the total RNAs were assessed by spectrophotometer (Nanodrop 1000, Nanodrop Technologies, Wilmington, DE, USA) and RNA integrity was verified using an Agilent 2100 bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled RNA was generated using the Agilent’s miRNA Complete Labeling and Hyb Kit with a sample input of 100 ng of total RNA.
|
| |
|
| Hybridization protocol |
Hybridization of labeled miRNA to 8x60K Human miRNA microarray slides (based on miRBase release 16.0) was for 20 hours at 55ºC on a rotator at 20 rpm.
|
| Scan protocol |
The slides were then washed and scanned with an Agilent microarray scanner.
|
| Description |
microRNA expression in patients with pre-diabetes
EP17
|
| Data processing |
Extracted data were analyzed with GeneSpring GX7.3 expression analysis software (Agilent Technologies).
|
| |
|
| Submission date |
Apr 23, 2015 |
| Last update date |
Apr 25, 2015 |
| Contact name |
Celine Latouche |
| E-mail(s) |
celine.latouche@bakeridi.edu.au
|
| Organization name |
Baker IDI Heart and Diabetes Institute
|
| Street address |
75 Commercial Road
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3004 |
| Country |
Australia |
| |
|
| Platform ID |
GPL16770 |
| Series (2) |
| GSE68224 |
MicroRNA signature in skeletal muscle in type 2 Diabetes [human] |
| GSE68226 |
MicroRNA signature in skeletal muscle in type 2 Diabetes and insulin resistant rats |
|
