|
| Status |
Public on Apr 25, 2015 |
| Title |
SkeletalMuscle_C [A75] |
| Sample type |
RNA |
| |
|
| Source name |
soleus_control
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain background: Sprague-Dawley
diet during pregnancy/lactation: control
offspring gender: male
offspring age: 1 year
tissue: skeletal muscle (soleus)
|
| Treatment protocol |
Rats: offspring from control dams vs offspring from dams fed a high fat diet during pregnancy and lactation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (including microRNAs) was extracted from frozen tissues using the TRIZOL reagent (Invitrogen) following the manufacturer’s recommended protocol. Concentration and purity of the total RNAs were assessed by spectrophotometer (Nanodrop 1000, Nanodrop Technologies, Wilmington, DE, USA) and RNA integrity was verified using an Agilent 2100 bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled RNA was generated using the Agilent’s miRNA Complete Labeling and Hyb Kit with a sample input of 100 ng of total RNA.
|
| |
|
| Hybridization protocol |
Hybridization of labeled miRNA to 8x15K Rat miRNA microarray slides (based on miRBase release 16.0) was for 20 hours at 55ºC on a rotator at 20 rpm.
|
| Scan protocol |
The slides were then washed and scanned with an Agilent microarray scanner.
|
| Description |
A75
microRNA expression in adult offspring from control dams
|
| Data processing |
Extracted data were analyzed with GeneSpring GX7.3 expression analysis software (Agilent Technologies).
|
| |
|
| Submission date |
Apr 23, 2015 |
| Last update date |
Apr 25, 2015 |
| Contact name |
Celine Latouche |
| E-mail(s) |
celine.latouche@bakeridi.edu.au
|
| Organization name |
Baker IDI Heart and Diabetes Institute
|
| Street address |
75 Commercial Road
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3004 |
| Country |
Australia |
| |
|
| Platform ID |
GPL14860 |
| Series (2) |
| GSE68225 |
MicroRNA signature in skeletal muscle in insulin resistant rats |
| GSE68226 |
MicroRNA signature in skeletal muscle in type 2 Diabetes and insulin resistant rats |
|
