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Sample GSM16815 Query DataSets for GSM16815
Status Public on Feb 18, 2004
Title 1hHypo_0hReox_3
Sample type RNA
 
Channel 1
Source name HUVEC with 1 hour hypoxia and no reoxygenation
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Human universal reference RNA (Stratagene, La Jolla, CA)
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description Human umbilical vein endothelial cells (HUVEC) were cultivated on 1%-gelatin-coated 75-cm2 culture flasks in Medium 200 supplemented with 2% fetal calf serum, penicillin (100 units/ml), streptomycin (100 units/ml) and Funizone (0.25 μg/ml) supplied by Cascade Biologics (Portland, OR). Prior to an experiment, HUVECs were subcultivated with Trypsin/EDTA onto 1% gelatin coated glass 30-mm glass coverslips in 60 mm glass Petri dishes.
 
Submission date Feb 11, 2004
Last update date Sep 08, 2006
Contact name Phillip Bowman
E-mail(s) phillip.bowman@cen.amedd.army.mil
Phone 210-916-4297
Organization name Institute of Surgical Research
Street address 3400 Rawley E. Chambers Ave
City San Antonio
State/province TX
ZIP/Postal code 78234-6315
Country USA
 
Platform ID GPL981
Series (1)
GSE1041 Responses of HUVEC to Hypoxia and Reoxygenation

Data table header descriptions
ID_REF
VALUE same as UNF_VALUE but with flagged values removed
F635_MEDIAN Cy5 channel median intensity of the spot.
F635_MEAN Cy5 channel average intensity of the spot.
F635_SD The standard deviation calculated for the Cy5 channel mean intensity of the spot.
B635_MEDIAN Cy5 channel background median intensity around the spot.
B635_MEAN Cy5 channel background average intensity around the spot.
B635_SD The standard deviation calculated for the Cy5 channel background average intensity around the spot.
F635_QUALITY Cy5 channel percentage of pixels in the spot more than one standard deviation above background.
F532_MEDIAN Cy3 channel median intensity of the spot.
F532_MEAN Cy3 channel average intensity of the spot.
F532_SD The standard deviation calculated for the Cy3 channel mean intensity of the spot.
B532_MEDIAN Cy3 channel background median intensity around the spot.
B532_MEAN Cy3 channel background average intensity around the spot.
B532_SD The standard deviation calculated for the Cy3 channel background average intensity around the spot.
F532_QUALITY Cy3 channel percentage of pixels in the spot more than one standard deviation above background.
FLAGS The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity.
UNF_VALUE log ratio of Median of Ratios between Cy5 and Cy3 channels. (log2 of Median of Ratios). The Median of Ratios is the median of the pixel-by-pixel ratios of pixel intensities that have had the median background intensity subtracted.

Data table
ID_REF VALUE F635_MEDIAN F635_MEAN F635_SD B635_MEDIAN B635_MEAN B635_SD F635_QUALITY F532_MEDIAN F532_MEAN F532_SD B532_MEDIAN B532_MEAN B532_SD F532_QUALITY FLAGS UNF_VALUE
1 0.349398064 760 761 202 160 178 150 97 677 704 179 152 166 70 98 0 0.349398064
2 0.229046123 139 147 57 130 148 154 2 173 181 49 136 145 52 32 -50 0.229046123
3 -0.287589516 191 198 80 120 129 54 58 270 283 115 132 140 52 80 0 -0.287589516
4 -0.236892274 4449 4435 1115 122 132 55 98 5904 6037 1531 130 141 69 98 0 -0.236892274
5 -0.291723315 3851 3409 1432 125 138 138 93 5366 4954 2074 135 151 141 94 0 -0.291723315
6 0.051164398 213 232 79 131 147 151 26 250 251 60 137 155 154 23 0 0.051164398
7 0.425653168 11877 11750 4752 132 144 68 99 10489 10270 4681 134 147 84 96 0 0.425653168
8 0.400252951 2450 2378 964 131 139 64 100 2147 2235 946 135 149 99 95 0 0.400252951
9 -0.04674019 1394 1324 403 141 153 64 97 1608 1596 573 139 149 59 97 0 -0.04674019
10 -0.053743577 427 435 117 134 143 57 98 502 531 119 136 145 69 100 0 -0.053743577
11 0.170152434 858 810 299 134 144 62 94 925 839 298 136 150 72 93 0 0.170152434
12 -0.423855206 295 317 107 152 161 91 77 371 369 75 142 159 81 97 0 -0.423855206
13 -3.182149309 603 612 152 144 154 71 100 4706 4978 1063 143 170 273 100 0 -3.182149309
14 -3.981850659 252 248 83 134 138 52 68 2494 2515 614 133 150 74 100 0 -3.981850659
15 -0.913370921 1321 1333 315 134 148 87 98 2699 2832 563 138 160 99 100 0 -0.913370921
16 -1.067853177 895 904 191 153 171 100 100 1933 1994 368 156 175 90 100 0 -1.067853177
17 0.342746623 1068 1022 319 143 153 69 98 1014 967 305 147 167 105 94 0 0.342746623
18 0.22036388 1102 1149 306 131 143 76 100 1061 1138 297 142 160 117 100 0 0.22036388
19 -1.078526678 212 224 82 133 145 91 42 382 383 132 143 160 94 85 0 -1.078526678
20 0.188074342 476 480 159 128 136 63 96 494 493 132 137 155 105 95 0 0.188074342

Total number of rows: 23232

Table truncated, full table size 1961 Kbytes.




Supplementary data files not provided
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