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Sample GSM16832 Query DataSets for GSM16832
Status Public on Feb 18, 2004
Title 1hHypo_12hReox_4
Sample type RNA
 
Channel 1
Source name HUVEC with 1 hour hypoxia and 12 hours reoxygenation
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Human universal reference RNA (Stratagene, La Jolla, CA)
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description Human umbilical vein endothelial cells (HUVEC) were cultivated on 1%-gelatin-coated 75-cm2 culture flasks in Medium 200 supplemented with 2% fetal calf serum, penicillin (100 units/ml), streptomycin (100 units/ml) and Funizone (0.25 μg/ml) supplied by Cascade Biologics (Portland, OR). Prior to an experiment, HUVECs were subcultivated with Trypsin/EDTA onto 1% gelatin coated glass 30-mm glass coverslips in 60 mm glass Petri dishes.
 
Submission date Feb 12, 2004
Last update date Sep 08, 2006
Contact name Phillip Bowman
E-mail(s) phillip.bowman@cen.amedd.army.mil
Phone 210-916-4297
Organization name Institute of Surgical Research
Street address 3400 Rawley E. Chambers Ave
City San Antonio
State/province TX
ZIP/Postal code 78234-6315
Country USA
 
Platform ID GPL981
Series (1)
GSE1041 Responses of HUVEC to Hypoxia and Reoxygenation

Data table header descriptions
ID_REF
VALUE same as UNF_VALUE but with flagged values removed
F635_MEDIAN Cy5 channel median intensity of the spot.
F635_MEAN Cy5 channel average intensity of the spot.
F635_SD The standard deviation calculated for the Cy5 channel mean intensity of the spot.
B635_MEDIAN Cy5 channel background median intensity around the spot.
B635_MEAN Cy5 channel background average intensity around the spot.
B635_SD The standard deviation calculated for the Cy5 channel background average intensity around the spot.
F635_QUALITY Cy5 channel percentage of pixels in the spot more than one standard deviation above background.
F532_MEDIAN Cy3 channel median intensity of the spot.
F532_MEAN Cy3 channel average intensity of the spot.
F532_SD The standard deviation calculated for the Cy3 channel mean intensity of the spot.
B532_MEDIAN Cy3 channel background median intensity around the spot.
B532_MEAN Cy3 channel background average intensity around the spot.
B532_SD The standard deviation calculated for the Cy3 channel background average intensity around the spot.
F532_QUALITY Cy3 channel percentage of pixels in the spot more than one standard deviation above background.
FLAGS The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity.
UNF_VALUE log ratio of Median of Ratios between Cy5 and Cy3 channels. (log2 of Median of Ratios). The Median of Ratios is the median of the pixel-by-pixel ratios of pixel intensities that have had the median background intensity subtracted.

Data table
ID_REF VALUE F635_MEDIAN F635_MEAN F635_SD B635_MEDIAN B635_MEAN B635_SD F635_QUALITY F532_MEDIAN F532_MEAN F532_SD B532_MEDIAN B532_MEAN B532_SD F532_QUALITY FLAGS UNF_VALUE
1 -0.249161539 506 535 233 220 240 124 77 514 514 195 142 164 326 60 0 -0.249161539
2 0.790009059 196 204 108 216 236 123 17 164 163 42 143 147 45 32 -50 0.790009059
3 0.2423974 255 299 192 218 245 139 19 203 392 1102 141 179 503 3 -50 0.2423974
4 -0.752903263 1652 1638 435 229 252 137 98 2468 2559 593 135 167 444 100 0 -0.752903263
5 -0.962063428 1031 1023 308 232 254 141 97 1649 1665 381 135 140 42 100 0 -0.962063428
6 0.704607749 300 302 133 223 244 140 28 225 221 66 143 151 73 53 -50 0.704607749
7 -0.29283201 2119 2112 754 219 237 124 98 2455 2408 646 135 142 63 100 0 -0.29283201
8 -0.142364516 766 767 310 233 243 127 91 720 713 223 129 133 39 96 0 -0.142364516
9 -0.549740313 571 571 254 220 240 128 79 714 699 185 141 146 56 98 0 -0.549740313
10 0.142525473 310 339 149 227 240 124 40 285 297 60 146 153 59 96 0 0.142525473
11 -0.398960361 470 501 260 239 250 135 67 450 453 122 137 142 44 97 0 -0.398960361
12 -0.173424812 309 308 159 244 252 130 26 253 252 48 137 145 47 90 -50 -0.173424812
13 -2.642306432 610 622 239 246 253 132 91 2339 2449 386 144 153 63 100 0 -2.642306432
14 -2.017345863 311 317 153 232 245 134 35 830 822 316 147 157 64 97 0 -2.017345863
15 -1.146981144 571 620 282 232 251 130 87 1013 999 224 148 156 63 100 0 -1.146981144
16 -1.232711018 489 512 242 239 258 138 70 866 855 298 154 160 59 95 0 -1.232711018
17 -0.173424812 404 417 187 222 243 136 59 406 406 128 155 161 60 93 0 -0.173424812
18 -0.330845336 478 500 217 224 251 148 70 483 485 137 157 166 80 97 0 -0.330845336
19 0.32916787 258 272 130 238 256 131 25 224 233 58 161 176 78 40 -50 0.32916787
20 0.013321273 338 328 162 253 276 130 31 294 294 87 184 208 93 59 0 0.013321273

Total number of rows: 23232

Table truncated, full table size 2009 Kbytes.




Supplementary data files not provided
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