|
| Status |
Public on May 15, 2015 |
| Title |
Spleen resident macrophage rep3 [140887_635] |
| Sample type |
RNA |
| |
|
| Source name |
Spleen resident macrophage rep3
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wild-type
phenotype: spleen resident
accession: FVB
|
| Growth protocol |
Metastasis associated macrophage were FACS sorted from mice bearing pulmonary metastasis of breast tumor cells, lung and spleen resident macrophages were sorted from healthy non-tumour bearing mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA was amplified with Ambion MessageAmp II aRNA kit (Invitrogen, Grand Island, NY, USA). RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer at the Einstein Genomic Core Facility
|
| Label |
Cy5
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
This sample is of FACS sorted macrophages associated with pulmonary metastasis of breast tumor from mouse treated with FLT1 specific inhibitory antibody (MF1). It is the third of three biological replicates used in this experiment, each from separate mouse.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
May 14, 2015 |
| Last update date |
May 15, 2015 |
| Contact name |
Bin-Zhi Qian |
| E-mail(s) |
binzhi.qian@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
MRC Centre for Reproductive Health
|
| Street address |
47 Little France Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH16 4TJ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13179 |
| Series (1) |
| GSE68862 |
Transcriptom analysis of differentially regulated genes in metastasis associated macrophages(MAM) compared with lung and spleen resident macrophages |
|
