|
| Status |
Public on Jan 01, 2016 |
| Title |
cardiomyocytes 5 day after proton radiation exposure rep2 |
| Sample type |
RNA |
| |
|
| Source name |
left ventricular murine cardiomyocytes to 90 cGy, 1 GeV proton (1H) 5 day after exposure
|
| Organism |
Mus musculus |
| Characteristics |
tissue: left ventricular murine cardiomyocytes
strain: C57Bl/6NT
Sex: male
age: adult (8-9 months old)
|
| Treatment protocol |
Irradiation and Dosimetry – Whole body irradiation of adult (8-9 months old) male C57Bl/6NT (Taconic) mice was performed at the NASA Space Radiation Laboratory (NSRL) at Brookhaven National Laboratory (Upton, NY). Using dose-rates between 0.05 Gy/min and 0.17 Gy/min, iron and proton, respectively; 0.15 Gy was delivered using 1 GeV/nucleon iron ions (LET ~151 keV per µm) or 0.9 Gy at 1 GeV energy protons (LET ~0.22 keV per µm).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cardiac Cell Isolation – Primary left ventricular cardiac cells from 8-9 months old C57Bl/6NT mice were isolated using standard preparation protocol that includes cannulation of the aorta and collagenase digestion followed by a Ca2+ gradient selection (22, 25, 26, 33). This procedure yielded >90% of Ca2+ tolerant cardiomyocytes (CMs). The resulting cell suspension could contain a small population of inflammatory and/or endothelial cells. Following isolation of CMs, RNA was extracted via TRIzol (Life Technologies, Grand Island, NY) according to the manufactures instructions. RNA quality and quantity was assessed using the 2100 Bioanalyzer (Agilent Tech., Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Follow manufacturor Affymetrix standard procedure
|
| Scan protocol |
Scanned with Affymetrix GeneChip imager
|
| Data processing |
MAS5.0 signal intensities analyzed using Dchip in Webarray
|
| |
|
| Submission date |
May 14, 2015 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Matthew Coleman |
| E-mail(s) |
coleman16@llnl.gov
|
| Organization name |
Lawrence Livermore National Laboratory
|
| Street address |
7000 East Ave.
|
| City |
Livermore |
| State/province |
CA |
| ZIP/Postal code |
94550 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE68875 |
Delayed Cardiomyocyte Response to Total Body Particle Radiation Exposure – Identification of Regulatory Gene Network [proton] |
| GSE68876 |
Delayed Cardiomyocyte Response to Total Body Particle Radiation Exposure - Identification of Regulatory Gene Network |
|
