|
| Status |
Public on Jan 11, 2016 |
| Title |
LNCap R1881 Rep #2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCap R1881 Rep #2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
|
| Treatment protocol |
LNCaP cells were transfected with siRNA against luciferase and GATA2, respectively. 72 h post-transfection, cells were cultured in phenol free RPMI +5%FCS+ 1% P/S + R1881 24hr, and then were harvested for RNA extraction.
|
| Growth protocol |
Cells were maintained in phenol free RPMI +5%FCS+ 1% P/S + R1881 24hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Trizol according to manufacturer's instructions. The quantity and quality of the isolated RNAs was determined using ND-1000 Spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Scan protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Description |
SAMPLE 10
|
| Data processing |
The data were processed and normalised using Illumina GenomeStudio
|
| |
|
| Submission date |
May 19, 2015 |
| Last update date |
Jan 11, 2016 |
| Contact name |
Jindan Yu |
| E-mail(s) |
jindan.yu@emory.edu
|
| Organization name |
Emory University
|
| Department |
Urology
|
| Lab |
Jindan Yu's lab
|
| Street address |
E330, 1760 Haygood Dr NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE69044 |
Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer [gene expression] |
| GSE69045 |
Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer |
|
