|
| Status |
Public on Jan 01, 2016 |
| Title |
48hpi mensenchyme, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse tracheal mesenchyme 48hrs post SO2 injury
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
tissue: trachea
cell type: mesenchyme
gender: male
treatment: 48hpi
|
| Treatment protocol |
Adult male C57Bl/6 mice were placed in individual compartments within a chamber and exposed to 500 ppm SO2 in air for 4 hr.
|
| Growth protocol |
Mouse tracheas were cleaned well, cut into pieces and incubated in Dispase at RT for 23min. Digestion was stopped by addition of DMEM with 5% FBS. Epithelium was peeled off with forcepsThe remaining total mesenchyme was used without further manipulation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Micro kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
|
| |
|
| Hybridization protocol |
Targets were hybridized to GeneChip® Mouse Genome 430 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned with Affymetrix GeneChip® Scanner 7G and AGCC software version 1.1
|
| Description |
Gene expression data of 48hpi mouse tracheal mesenchyme replicate 2
|
| Data processing |
Affymetrix Expression Console version 1.1 was used to generate the .cel files. Partek Genomics Suite 6.5 (Partek Inc., St. Louis, MO) was used to perform data analysis. Robust multi-chip analysis (RMA) normalization was done on the entire data set.
|
| |
|
| Submission date |
May 19, 2015 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Xia Gao |
| E-mail(s) |
xia.gao@dm.duke.edu
|
| Organization name |
Duke University
|
| Street address |
353 Nanaline Duke Bldg. Research Dr.
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE69057 |
Gene expression data from mouse tracheal mesenchymal cells before and 48hrs after SO2 injury |
| GSE69058 |
Gene expression data from mouse tracheal cells before and 48hrs after SO2 injury |
|
