|
| Status |
Public on Jun 01, 2015 |
| Title |
Ncoa3 shRNA #1 |
| Sample type |
RNA |
| |
|
| Source name |
Neurons expressing Ncoa3 shRNA from 1st experiment
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: neuron
treatment: Ncoa3 shRNA
|
| Treatment protocol |
After 4 days in vitro the neurons were infected with 1.8*10^6 IFU/ml adeno-associated virus (AAV) expressing either control shRNA (samples 1, 3, 5) or Ncoa3 shRNA (samples 2, 4, 6).
|
| Growth protocol |
Hippocampi of E18 rats were dissected and cultured in the presence of 10 µM of 5-fluorodeoxyuridine (FUDR).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated after 14 div using Mirvana-Kit and subjected to this microarray analysis.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol. According to standard procedure of the Genecore Facility at EMBL Heidelberg.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol. According to standard procedure of the Genecore Facility at EMBL Heidelberg.
|
| Scan protocol |
Standard Affymetrix protocol. According to standard procedure of the Genecore Facility at EMBL Heidelberg.
|
| Data processing |
Expression Console (Affymetrix) and GeneSpring (Agilent).
|
| |
|
| Submission date |
May 21, 2015 |
| Last update date |
Jun 01, 2015 |
| Contact name |
Peter Harry Störchel |
| E-mail(s) |
peter.stoerchel@staff.uni-marburg.de
|
| Organization name |
Philipps-Universität Marburg
|
| Department |
Institut für Physiologische Chemie
|
| Lab |
Schratt
|
| Street address |
Karl-von-Frisch-Str. 1
|
| City |
Marburg |
| ZIP/Postal code |
35032 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE69131 |
Gene Expression of primary rat hippocampal neurons after Ncoa3 knockdown |
|
