|
| Status |
Public on May 28, 2015 |
| Title |
axil_9day_non-shaded 1 |
| Sample type |
RNA |
| |
|
| Source name |
leaf axil, non-shaded
|
| Organism |
Populus tremula x Populus tremuloides |
| Characteristics |
clone: T89
treatment: 9 days after dark induction
|
| Treatment protocol |
For dark-induction experiments, fully expanded leaves with a petiolar angle of 75 ° to 90 ° from 1.5 m to 2 m tall trees were selected. Leaf blades were covered with aluminum bags for 9 days under standard greenhouse conditions. Control samples were bagged in transparent plastic bags of the same weight.
|
| Growth protocol |
Temperature was 22/18 °C (light/dark), photoperiod was 16 h, and lightTemperature was 22/18 °C (light/dark), photoperiod was 16 h, and light intensity was 90 μmol·m-2·s-1 Temperature was 22/18 °C (light/dark), photoperiod was 16 h, and light intensity was 90 μmol·m-2·s-1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 3-mm-long petioles near the junction to the stem using RNeasy Plant Mini Kit (Qiagen). spectrophotometer
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression in leaf axil, 9 days after dark induction
|
| Data processing |
Robin affymetrix data analysis summary, normalization method: rma, P-value correction method: BH, analysis strategy: Limma, Multiple testing strategy: nestedF P-value cut-off value for significant differential expression: 0.05 Genes that showed a log2-fold change smaller than two ignored: yes
|
| |
|
| Submission date |
May 27, 2015 |
| Last update date |
May 28, 2015 |
| Contact name |
Urs Fischer |
| E-mail(s) |
urs.fischer@slu.se
|
| Phone |
0046907868411
|
| Organization name |
Umeå Plant Science Centre
|
| Department |
Forest Genetics and Plant Physiology
|
| Lab |
Fischer
|
| Street address |
Swedish Agricultural University
|
| City |
Umeå |
| State/province |
Schweden |
| ZIP/Postal code |
90183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE69277 |
Gene expression during dark-induced leaf abscission in Populus x tremula |
|
