|
Status |
Public on May 30, 2015 |
Title |
HGPS_patient_1 |
Sample type |
RNA |
|
|
Source name |
Human skin fibroblasts from HGPS patient 1
|
Organism |
Homo sapiens |
Characteristics |
sample type: HGPS patient tissue: Primary in vitro culture human skin fibroblasts
|
Treatment protocol |
Cells were not treated
|
Growth protocol |
All cell lines were grown in vitro using MEM tissue culture medium supplemented with 15% foetal bovine serum, L-glutamine, non-essential amino acids, pyruvate as well as pen/strep.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from cells was harvested using the Qiagen RNA isolation kit according to the manufecturers instructions.
|
Label |
Biotinylated
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
|
|
Hybridization protocol |
Fragmentation and hybridization was performed according to standard Affymetric guidelines for the HG-U133_plus_2 arrays
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
mRNA gene expression data from human skin fibroblasts
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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|
|
Submission date |
May 29, 2015 |
Last update date |
May 30, 2015 |
Contact name |
Nard Kubben |
Organization name |
NCI, NIH
|
Street address |
41 Library Drive
|
City |
Bethesda |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE69391 |
Expression data from young and old healthy humans, as well as HGPS patients |
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