|
| Status |
Public on May 30, 2015 |
| Title |
HGPS_patient_3 |
| Sample type |
RNA |
| |
|
| Source name |
Human skin fibroblasts from HGPS patient 3
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: HGPS patient
tissue: Primary in vitro culture human skin fibroblasts
|
| Treatment protocol |
Cells were not treated
|
| Growth protocol |
All cell lines were grown in vitro using MEM tissue culture medium supplemented with 15% foetal bovine serum, L-glutamine, non-essential amino acids, pyruvate as well as pen/strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from cells was harvested using the Qiagen RNA isolation kit according to the manufecturers instructions.
|
| Label |
Biotinylated
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Fragmentation and hybridization was performed according to standard Affymetric guidelines for the HG-U133_plus_2 arrays
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
mRNA gene expression data from human skin fibroblasts
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 29, 2015 |
| Last update date |
May 30, 2015 |
| Contact name |
Nard Kubben |
| Organization name |
NCI, NIH
|
| Street address |
41 Library Drive
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE69391 |
Expression data from young and old healthy humans, as well as HGPS patients |
|
