|
| Status |
Public on Jun 04, 2015 |
| Title |
SN10-0821 |
| Sample type |
RNA |
| |
|
| Source name |
patients with sepsis caused by other pathogens
|
| Organism |
Homo sapiens |
| Characteristics |
pathogens: Staphylococcus aureus
study group: Other sepsis
tissue: Whole blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole blood lysate using the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Life Technologies, CA) according to the manufacturer’s instructions. Quality control was performed with the Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina labeling protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Arrays were scanned using the Illumina HiScan and the standard Illumina scanning protocol.
|
| Description |
SN10.0821_Ot
|
| Data processing |
The data were preprocessed using R, which included the following steps: quantile normalization, flooring all intensities <10 to 10, and log2 transformation. PALO (Presence in At least One), which selects only transcripts with detection p-values < 0.01 in at least one sample, was performed to reduce background noise.
|
| |
|
| Submission date |
Jun 03, 2015 |
| Last update date |
Jun 04, 2015 |
| Contact name |
Scott Presnell |
| E-mail(s) |
SPresnell@benaroyaresearch.org
|
| Organization name |
Benaroya Research Institute
|
| Street address |
1201 Ninth Avenue
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE69528 |
A Blood Transcriptional Diagnostic Assay for Septicemic Melioidosis |
|
