|
| Status |
Public on Jun 11, 2015 |
| Title |
MetI |
| Sample type |
RNA |
| |
|
| Source name |
Wheat straw culture of T. reesei with L-methionine
|
| Organism |
Trichoderma reesei QM9414 |
| Characteristics |
added gene repressing substance: 100µM L-methionine
|
| Treatment protocol |
After 48 hours, L-methionine resulting in a final concentration of 100µM was added
|
| Growth protocol |
Cultures of T. reesei QM9414 (ATCC26921) were pregrown for 48 hours on Mandels Andreotti Medium with pretreated wheat straw as the sole carbon source as previously described (Bischof et. al., Biotechnology for Biofuels 2013, 6:127)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen, Hilden, Germany) and purified using RNeasy MinElute Kit (Qiagen, Hilden, Germany)
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
Sample with added L-methionine
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Jun 10, 2015 |
| Last update date |
Jun 11, 2015 |
| Contact name |
Robert Bischof |
| E-mail(s) |
robert.bischof@acib.at
|
| Phone |
+43 01 58801 166476
|
| Organization name |
ACIB GmbH
|
| Street address |
Gumpendorferstraße 1a
|
| City |
Vienna |
| ZIP/Postal code |
1060 |
| Country |
Austria |
| |
|
| Platform ID |
GPL10642 |
| Series (1) |
| GSE69745 |
Analysis of wheat straw grown Trichoderma reesei gene expression as influenced by L-methionine in the medium |
|
