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Status |
Public on Aug 11, 2015 |
Title |
0-4mm, biological replicate 1 |
Sample type |
RNA |
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Source name |
Seeds of Glycine max at developmental stage (0-4mm)
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Organism |
Glycine max |
Characteristics |
cultivar: Pusa 16 developmental stage: 0-4mm tissue: seed
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Treatment protocol |
Seeds of Glycine max grown at 28/26°C, 12h light/dark were collected at different developmental stages(0-4, 4-8, 8-12 and 12-16 mm) and analyzed.
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Growth protocol |
Seeds of Glycine max at developmental stages are utilized for the study
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix Soybean Genome Array
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Scan protocol |
Genechips were scanned using affymetrix scanner type M10
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Description |
Gene expression data from seeds of Glycine max at developmental stage 0-4mm SAMPLE_1_R1
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Data processing |
Probe level data was summarized through RMA algorithm, and summarized data was used for differential expression analysis
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Submission date |
Jun 12, 2015 |
Last update date |
Aug 11, 2015 |
Contact name |
Archana Sachdev |
E-mail(s) |
arcs_bio@yahoo.com
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Organization name |
Indian Agricultural Research Institute
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Department |
Biochemistry
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Street address |
Pusa campus
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City |
New Delhi |
State/province |
NCR Delhi |
ZIP/Postal code |
110012 |
Country |
India |
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Platform ID |
GPL13674 |
Series (1) |
GSE69821 |
Transcriptome analysis during seed development provides insights into altered gene expression in Glycine max |
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