|
| Status |
Public on Aug 11, 2015 |
| Title |
4-8mm, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Seeds of Glycine max at developmental stage (4-8mm)
|
| Organism |
Glycine max |
| Characteristics |
cultivar: Pusa 16
developmental stage: 4-8mm
tissue: seed
|
| Treatment protocol |
Seeds of Glycine max grown at 28/26°C, 12h light/dark were collected at different developmental stages(0-4, 4-8, 8-12 and 12-16 mm) and analyzed.
|
| Growth protocol |
Seeds of Glycine max at developmental stages are utilized for the study
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix Soybean Genome Array
|
| Scan protocol |
Genechips were scanned using affymetrix scanner type M10
|
| Description |
Gene expression data from seeds of Glycine max at developmental stage 4-8mm
SAMPLE_2_R3
|
| Data processing |
Probe level data was summarized through RMA algorithm, and summarized data was used for differential expression analysis
|
| |
|
| Submission date |
Jun 12, 2015 |
| Last update date |
Aug 11, 2015 |
| Contact name |
Archana Sachdev |
| E-mail(s) |
arcs_bio@yahoo.com
|
| Organization name |
Indian Agricultural Research Institute
|
| Department |
Biochemistry
|
| Street address |
Pusa campus
|
| City |
New Delhi |
| State/province |
NCR Delhi |
| ZIP/Postal code |
110012 |
| Country |
India |
| |
|
| Platform ID |
GPL4592 |
| Series (1) |
| GSE69821 |
Transcriptome analysis during seed development provides insights into altered gene expression in Glycine max |
|
