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Sample GSM1709513 Query DataSets for GSM1709513
Status Public on Aug 11, 2015
Title 12-16mm, biological replicate 2
Sample type RNA
 
Source name Seeds of Glycine max at developmental stage (12-16mm)
Organism Glycine max
Characteristics cultivar: Pusa 16
developmental stage: 12-16mm
tissue: seed
Treatment protocol Seeds of Glycine max grown at 28/26°C, 12h light/dark were collected at different developmental stages(0-4, 4-8, 8-12 and 12-16 mm) and analyzed.
Growth protocol Seeds of Glycine max at developmental stages are utilized for the study
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix Soybean Genome Array
Scan protocol Genechips were scanned using affymetrix scanner type M10
Description Gene expression data from seeds of Glycine max at developmental stage 12-16mm
SAMPLE_4_R2
Data processing Probe level data was summarized through RMA algorithm, and summarized data was used for differential expression analysis
 
Submission date Jun 12, 2015
Last update date Aug 11, 2015
Contact name Archana Sachdev
E-mail(s) arcs_bio@yahoo.com
Organization name Indian Agricultural Research Institute
Department Biochemistry
Street address Pusa campus
City New Delhi
State/province NCR Delhi
ZIP/Postal code 110012
Country India
 
Platform ID GPL13674
Series (1)
GSE69821 Transcriptome analysis during seed development provides insights into altered gene expression in Glycine max

Data table header descriptions
ID_REF
VALUE RMA normalized signal

Data table
ID_REF VALUE
11764300 2.860302634
11764307 2.025412186
11764329 3.844801771
11764340 3.217432109
11764348 2.399978331
11764350 3.814184599
11764369 5.371777502
11764376 2.162423299
11764381 3.701165538
11764389 3.23911969
11764394 3.103470883
11764400 2.808712782
11764407 3.956937453
11764411 3.809795689
11764418 3.213691853
11764420 7.232141719
11764429 3.296161788
11764436 2.178780556
11764440 3.517542335
11764445 2.76286676

Total number of rows: 67740

Table truncated, full table size 1381 Kbytes.




Supplementary file Size Download File type/resource
GSM1709513_LS_2.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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