|
| Status |
Public on Jun 15, 2007 |
| Title |
Clone concentration experiment: Sample 2: Scan 1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila cRNA transcripts at known concentrations hybridized to the array in the absense of complex background.
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Wash Condition: 1 cycle of non-stringent wash
Hybridization Time: 16 hrs
Labeling Condition: 1 nM labeled spikes, no background
|
| Extracted molecule |
total RNA |
| Extraction protocol |
4 cDNAs from the Drosophila Gene Collection adult testes (AT) library were PCR amplified, pooled, and used directly as targets for in vitro transcription (IVT).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to standard Affymetrix protocol, following the synthesis and purification of labeled cRNAs the product is fragmented and hybridized to the GeneChip for 16 hours, after which the array is subject to a modified non-stringent washing and staining steps prior to being scanned to obtain hybridization intensities.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner.
|
| Description |
Raw probe intensity data (PM) corresponding to spiked targets.
|
| Data processing |
Raw probe intensities.
|
| |
|
| Submission date |
Feb 22, 2007 |
| Last update date |
Jun 14, 2007 |
| Contact name |
Diana Abdueva |
| Organization name |
USC
|
| Street address |
443 S San Pedro St, STE 403
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90013 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE7110 |
Explaining Differences in Saturation Levels for Affymetrix GeneChip Arrays |
|
