|
| Status |
Public on Jun 29, 2015 |
| Title |
LTED+AZD5363 replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
compound: LTED+AZD5363- Mutant PIK3CA, resistant to estrogen deprivation, treated with AKT inhibitor (AZD5363)
cell line: MCF7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA from treated cells was extracted with RNeasy Mini Kit (Qiagen), as per the manufacturer's instructions, and quantification performed using the Agilent 2100 Bioanalyzer (Expert Software version B.02.03) with RNA Nano LabChip Kits (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were transformed and normalized using variance-stabilizing transformation and robust spline normalization method in the Lumi package in R
|
| |
|
| Submission date |
Jun 15, 2015 |
| Last update date |
Jun 29, 2015 |
| Contact name |
Alice Gao |
| E-mail(s) |
alice.gao@icr.ac.uk
|
| Organization name |
The Institute of Cancer Research
|
| Department |
Breast Cancer Now
|
| Lab |
Molecular Cell Biology
|
| Street address |
237 Fulham Road
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
SW3 6JB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE69893 |
AKT antagonist AZD5363 influences estrogen receptor function in endocrine resistant breast cancer and synergizes with fulvestrant (ICI182780) in vivo |
|
