|
| Status |
Public on Jun 19, 2015 |
| Title |
T_61 reanalysis |
| Sample type |
RNA |
| |
|
| Source name |
Breast tumor sample
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: invasive breast carcinoma sample
molecular subtype: HER2+
|
| Treatment protocol |
No
|
| Growth protocol |
Tumor samples were obtained from patients treated at the hospital of Institut Curie (Biological Resource Center, Paris, France). Healthy samples were obtained from mammary plastic surgery. Cell lines were grown from the American Type Culture Collection (ATCC),
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified from frozen tumor samples using the RNeasy Mini Kit (Qiagen) followed by the RNA clean up kit (Macherey Nagel), according to manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Following RNA quality and quantity control, 2 µg of total RNA were amplified and labeled according to the Affymetrix one cycle synthesis target preparation protocol to hybridize Affymetrix U133 plus 2.0 chips, as described by the company.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Gene expression data for invasive breast carcinoma: reanalysis GSM1589747
|
| Data processing |
Data were analyzed using standard AffyCDF or Brainarray HGU133Plus2_Hs_ENTREZG version 13 custom chipset definition file. Data were normalized using Brainarray.
Gene summarized expression value obtained using Brainarray CDF and software
|
| |
|
| Submission date |
Jun 18, 2015 |
| Last update date |
Jun 19, 2015 |
| Contact name |
Tatiana Popova |
| Organization name |
Institute Curie
|
| Street address |
26, rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75248 |
| Country |
France |
| |
|
| Platform ID |
GPL16311 |
| Series (1) |
| GSE69986 |
Expression profiling of invasive breast carcinoma samples from Institut Curie |
|
| Relations |
| Reanalysis of |
GSM1589747 |
