|
Status |
Public on Jul 23, 2015 |
Title |
bKO [S05089] |
Sample type |
SRA |
|
|
Source name |
Pro-B cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: Bone marrow genotype: Cnot3(fl/fl)Mb1(cre/+)
|
Treatment protocol |
Pro-B cells (Lin-B220+IgM-CD43+CD25-CD19+) were FACS-sorted from six mice and pooled for each genotype.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Invitrogen). TruSeq RNA sample preparation kit v2
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Data processing |
Read alignment: TopHat v.2.0.13 with default settings expression level: Cufflinks v.2.2.1 with default settings Genome_build: mouse NCBI build 37.2
|
|
|
Submission date |
Jun 19, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Osamu Ohara |
E-mail(s) |
ohara@kazusa.or.jp
|
Phone |
+81-438-52-3913
|
Organization name |
Kazusa DNA Research Institute
|
Department |
Technology Development
|
Lab |
Human DNA Analysis
|
Street address |
2-6-7 Kazusa-Kamatari
|
City |
Kisarazu |
State/province |
Chiba |
ZIP/Postal code |
292-0818 |
Country |
Japan |
|
|
Platform ID |
GPL18480 |
Series (1) |
GSE64455 |
Regulation of B cell early development by CNOT3 |
|
Relations |
BioSample |
SAMN03782918 |
SRA |
SRX1066206 |