|
| Status |
Public on Jul 23, 2015 |
| Title |
bKO [S05089] |
| Sample type |
SRA |
| |
|
| Source name |
Pro-B cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Bone marrow
genotype: Cnot3(fl/fl)Mb1(cre/+)
|
| Treatment protocol |
Pro-B cells (Lin-B220+IgM-CD43+CD25-CD19+) were FACS-sorted from six mice and pooled for each genotype.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Invitrogen).
TruSeq RNA sample preparation kit v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Read alignment: TopHat v.2.0.13 with default settings
expression level: Cufflinks v.2.2.1 with default settings
Genome_build: mouse NCBI build 37.2
|
| |
|
| Submission date |
Jun 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Osamu Ohara |
| E-mail(s) |
ohara@kazusa.or.jp
|
| Phone |
+81-438-52-3913
|
| Organization name |
Kazusa DNA Research Institute
|
| Department |
Technology Development
|
| Lab |
Human DNA Analysis
|
| Street address |
2-6-7 Kazusa-Kamatari
|
| City |
Kisarazu |
| State/province |
Chiba |
| ZIP/Postal code |
292-0818 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE64455 |
Regulation of B cell early development by CNOT3 |
|
| Relations |
| BioSample |
SAMN03782918 |
| SRA |
SRX1066206 |
