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Sample GSM1720852 Query DataSets for GSM1720852
Status Public on Jun 25, 2015
Title soleus-cKO-2
Sample type RNA
 
Source name soleus muscle
Organism Mus musculus
Characteristics tissue: soleus
genotype: nebulin KO
Sex: male
age: 41 days old
Treatment protocol Mice are homozygous for the conditional nebulin allele and are hemizygous for the muscle-specific MCK-Cre transgene (cKO) or lack the MCK-Cre transgene (Control).
Growth protocol Mice are on a mixed C57BL/6J and 1219S6/SvEvTac background. Mice are grown in a pathogen-free barrier facility with 12hr light:12hr dark cycle and are provided water and Teklad Global Rodent Diet (2919) ad libitum.
Extracted molecule total RNA
Extraction protocol Tissues were stored in Invitrogen RNAlater following manufacturer's protocol. Total RNA was isolated using Qiagen RNeasy fibrous tissue kit following the manufacturer's protocol.
Label biotin
Label protocol Samples were biotin-labelled using the Affymetrix GeneChip WT Plus reagant per manufacturer's protocol.
 
Hybridization protocol Samples were hybridized at 45C 17hours using the Affymetrix GeneChip Hybridization Buffers in with the Affymetrix Hyb Oven 645 and Fluidics Station 450.
Scan protocol The Affymetrix Scanner 3000 7G was used to scan the GeneChips using Affymetrix GeneChip Console.
Description nebulin-deficient soleus
Data processing Affymetrix Expression Console used RMA default settings and MoGene-1_0-st-v1.na34.mm10.transcript.csv annotation file. Affymetrix Transcript Analysis Console was used with One-Way Between-Subject ANOVA (Unpaired) and default filter criteria: Fold Change (linear) < -2 or Fold Change (linear) > 2 and ANOVA p-value (Condition pair) < 0.05.
 
Submission date Jun 24, 2015
Last update date Jun 25, 2015
Contact name Henk L Granzier
E-mail(s) granzier@email.arizona.edu
Organization name University of Arizona
Street address 1656 E Mabel St.
City Tucson
State/province AZ
ZIP/Postal code 85724
Country USA
 
Platform ID GPL6246
Series (1)
GSE70213 The effect of Nebulin-Deficiency on Skeletal Muscle

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10344614 38.19243
10344616 2.861815
10344620 5.515815
10344622 58.25288
10344624 358.2584
10344633 390.0673
10344637 90.74055
10344653 5.632401
10344658 153.0414
10344674 5.603591
10344679 11.67739
10344707 243.2217
10344713 203.624
10344715 4.375034
10344717 16.76994
10344719 34.44699
10344721 2.64296
10344723 45.88184
10344725 118.7894
10344741 532.1091

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM1720852_cKOSoleus2.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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