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Sample GSM1721144 Query DataSets for GSM1721144
Status Public on Jun 25, 2015
Title WT batch (DNA)
Sample type SRA
 
Source name WCH_DNA
Organism Staphylococcus aureus
Characteristics strain: WCH-SK2
phenotype: MRSA
medium: batch culture - TSB
stress: no stress
Treatment protocol Cells were harvested and treated with RNA protect (Qiagen)
Growth protocol Strain WCH-SK2 was cultured in batch culture condition and in a prologned continuous culture (chemostat) for 60 days
Extracted molecule genomic DNA
Extraction protocol Whole genomic DNA extraction and purification were performed according to manufacturers protocols (Blood and Cell culture DNA Maxi kit PN13362 and QIAGEN Genomic-tip 500/G columns, QIAGEN
SMRT sequencing from sequencing service provider (DNA Link, South Korea).
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model PacBio RS II
 
Data processing Library strategy: DNA-seq
Bowtie2 to align reads from samples
Genome_build: NC_007795; WCH-SK2 batch and WCH-SK2 SCV sequence
Supplementary_files_format_and_content: excel files (comma seperated values files) and excel worksheet files; fasta files are available on the series record.
 
Submission date Jun 24, 2015
Last update date May 15, 2019
Contact name Long M.G Bui
E-mail(s) bmglongd99@gmail.com
Phone 06183132610
Organization name University of Adelaide
Department Microbiology
Lab 416
Street address North Terrace Campus
City Adelaide
State/province South Australia
ZIP/Postal code 5005
Country Australia
 
Platform ID GPL20613
Series (1)
GSE63750 Prolonged growth of a clinical S. aureus strain selects for a stable SCV and Full genomic characterization of the development in a clinical Staphylococcus aureus strain of a stable Small Colony Variant cell-type
Relations
BioSample SAMN03792421
SRA SRX1074085

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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