|
| Status |
Public on Jul 30, 2007 |
| Title |
Female wildtype/Male wildtype #2 from STAT5a KO study |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
frozen adult male mouse liver tissue prepared using Trizol
|
| Organism |
Mus musculus |
| Characteristics |
Strain: 129JxBlack Swiss; Gender: male; Tissue: liver; Age: 8-9 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction
|
| Label |
Cy3
|
| Label protocol |
amino-allyl reverse transcription
|
| |
|
| Channel 2 |
| Source name |
frozen adult female mouse liver tissue prepared using Trizol
|
| Organism |
Mus musculus |
| Characteristics |
Strain: 129JxBlack Swiss; Gender: female; Tissue: liver; Age: 8-9 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction
|
| Label |
Cy5
|
| Label protocol |
amino-allyl reverse transcription
|
| |
|
| |
| Hybridization protocol |
Final volume of 3 ml in a flexible plastic enclosure at 40°C on a rotating platform in a hybridization oven. After hybridization for 16−24 h, slides were washed (rocking approx 30s in 6x SSPE, 0.005% sarcosine, then rocking approx 30s in 0.06x SSPE). Protocol taken from PMID: 11283592
|
| Scan protocol |
confocal laser scanner (General Scanning, Wilmington, MA; Genetic Microsystems, Woburn, MA; or Axon, Foster City, CA) Protocol taken from PMID: 11283592
|
| Description |
The genetic expression profile for a single male and single female is given as a normalized ratio (female over male) as reported from Rosetta Resolver. The reported ratio is the average of two dye-swapped fluorescent reverse pairings. The purpose of this comparison is to highlight sex-specific differences in mouse liver gene expression.
|
| Data processing |
Rosetta Resolver pipeline
|
| |
|
| Submission date |
Mar 01, 2007 |
| Last update date |
Jul 30, 2007 |
| Contact name |
David J. Waxman |
| E-mail(s) |
djw@bu.edu
|
| Organization name |
Boston University
|
| Department |
Department of Biology and Bioinformatics Program
|
| Street address |
5 Cummington Mall
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL3562 |
| Series (2) |
| GSE7169 |
Sex-dependent and STAT5a-dependent Liver Gene Expression |
| GSE7170 |
Sex-dependent and strain-dependent gene liver gene expression |
|
