|
| Status |
Public on Jan 28, 2016 |
| Title |
P2_MRD |
| Sample type |
genomic |
| |
|
| Source name |
Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
tumor: Multiple myeloma
sample type: Minimal Residual Disease
cell type: Clonal Plasma Cells
|
| Treatment protocol |
A total of 40 elderly, transplant-ineligible patients with newly-diagnosed symptomatic MM staged according to the International Myeloma Working Group criteria 24 were prospectively studied after inclusion in the GEM2010MAS65 trial. In all patients, bone marrow (BM) aspirates were collected at diagnosis and after 9 cycles of induction therapy. In brief, patients treatment consisted of bortezomib, melphalan, prednisone (VMP) and lenalidomide plus low-dose dexamethasone (Rd), given in a sequential (n=21) or alternating (n=19) manner.
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was digested with Nsp I and Sty I restriction enzymes, ligated to adapters with T4 DNA ligase and amplified. PCR products were concentrated and fragmented (DNAsal), and then end-labeled with deoxynucleotidil transferase
|
| Label |
biotin
|
| Label protocol |
Labelling and hybridizations were performed according to protocols from Affymetrix
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
Multiple Myeloma Plasma Cells. Sample 6. Minimal Residual Disease.
|
| Data processing |
primary data description: Weighted Log2 Ratios from ChAS console
|
| |
|
| Submission date |
Jun 30, 2015 |
| Last update date |
Jan 28, 2016 |
| Contact name |
Bruno Paiva |
| E-mail(s) |
bpaiva@unav.es
|
| Organization name |
Clinica Universidad de Navarra
|
| Street address |
Av. Pio XII, 55
|
| City |
Pamplona |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL18637 |
| Series (2) |
| GSE70397 |
Phenotypic and genomic analysis of multiple myeloma minimal residual disease clonal plasma cells: a new model to understand chemoresistance [CytoScan750K Copy Number, LOH] |
| GSE70399 |
Phenotypic and genomic analysis of multiple myeloma minimal residual disease clonal plasma cells: a new model to understand chemoresistance |
|
