|
| Status |
Public on Sep 30, 2007 |
| Title |
Kidney Cortex, 10 week old male rat, diabetic (Kidney_10WOM_DM_Rep2) |
| Sample type |
RNA |
| |
|
| Source name |
Kidney Cortex, 10 week old male rat, diabetic
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sprague Dawley rat, male, diabetic from 4-10 weeks of age; kidney cortex
|
| Biomaterial provider |
Pascale H Lane
|
| Treatment protocol |
Injected with streptozocin, 65 mg/kg iv, at 4 weeks of age; insulin palmitate pellet placed 3 days later; ad lib food and water; blood glucose 350-450 mg/dl for 6 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Aurum Total RNA Mini Kit (BioRAd Laboratories, Hercules, CA)
|
| Label |
Biotin
|
| Label protocol |
cRNA probes were biotinylated in a core facility
|
| |
|
| Hybridization protocol |
Per Affymetrix instructions
|
| Scan protocol |
GeneChip Scanner 3,000 6G per Affymetrix instructions
|
| Description |
All labeling, hybridization, and scanning were performed in the microarray core facility of the University of Nebraska Medical Center
|
| Data processing |
Images were analyzed using GCOS imaging software. Quality metric parameters including noise level, background, and the efficiency of reverse transcription were ascertained for all hybridizations. Data sets passing the stringent quality recommendations were normalized using the GCOS software, and the raw intensity values exported for further analysis
|
| |
|
| Submission date |
Mar 13, 2007 |
| Last update date |
Mar 13, 2007 |
| Contact name |
Pascale Hammond Lane |
| E-mail(s) |
phlane@unmc.edu
|
| Phone |
402-559-6055
|
| Fax |
402-559-8033
|
| Organization name |
University of Nebraska
|
| Department |
Pediatrics
|
| Lab |
DRC 6032
|
| Street address |
982169 Nebraska Medical Center
|
| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198-2169 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE7253 |
Puberty and Diabetes in the Kidney |
|
