|
| Status |
Public on Aug 09, 2007 |
| Title |
Donor A, post G-CSF |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood, prior to G-CSF
|
| Organism |
Homo sapiens |
| Characteristics |
no additional information
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RBC lysis with ammonium chloride Buffer EL, followed by RNA column purification
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45degC onto HG U133 Plus 2.0 GeneChips. Gene Chips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3005
|
| Description |
Donor A, after G-CSF
|
| Data processing |
The data were analyzed with GeneChip Operating Software Version 1.2, using Affymetrix default analysis settings, a target value of 500 and a normalization value of 1.
|
| |
|
| Submission date |
Mar 29, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Matthew Buzzeo |
| E-mail(s) |
mbuzzeo@health.usf.edu
|
| Organization name |
University of South Florida College of Medicine
|
| Street address |
12901 Bruce B. Downs Blvd
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7400 |
Hematopoietic stem cell mobilization with G-CSF (gene expression profiling) |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
