|
| Status |
Public on Dec 18, 2008 |
| Title |
Primary rat spinal cord astrocytes NonTG-B |
| Sample type |
RNA |
| |
|
| Source name |
Spinal cord astrocytes NonTG
|
| Organism |
Rattus norvegicus |
| Characteristics |
Primary spinal cord astrocytes from non-transgenic 1 day old rat littermates obtained from the breeding of a hSOD1G93A positive male with a negative female.
Astrocytes were prepared as described in Cassina P, et al.J Neurosci Res. 2002;67(1):21-9.
Transgenic hSOD1G93A rats were provided by D. S. Howland (Howland et al. 2002; PNAS, 99(3): 1604-1609)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted with RNeasy Mini (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
|
| |
|
| Hybridization protocol |
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
|
| Scan protocol |
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
|
| Description |
Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
5 ug of total RNA were used for the amplification.
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
|
| Data processing |
MAS5.0 (GCOS 1.4)
Alpha1 (0.05000000); Alpha2 (0.06500000); Tau (0.01500000); TGT (500)
|
| |
|
| Submission date |
Apr 03, 2007 |
| Last update date |
Dec 18, 2008 |
| Contact name |
Marcelo R. Vargas |
| E-mail(s) |
vargasm@iibce.edu.uy
|
| Organization name |
Instituto Clemente Estable
|
| Department |
Neurobiología Celular y Molecular
|
| Street address |
Av. Italia 3318
|
| City |
Montevideo |
| ZIP/Postal code |
11600 |
| Country |
Uruguay |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE7441 |
Transcriptional profile of primary astrocytes expressing ALS-linked mutant SOD1. |
|
