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Sample GSM180284 Query DataSets for GSM180284
Status Public on Oct 31, 2008
Title ABRO_OAR27K-508_9-9-05
Sample type RNA
 
Channel 1
Source name REF
Organism Arabidopsis thaliana
Characteristics Centaurea maculosa rosette leaf
seed source: Missoula Montana, plant ~6 months old
Biomaterial provider Seeds obtained from Callaway at the Unviersity of MT, Missoula
Growth protocol Invasive Centaurea maculosa plants from Missoula, MT were grown from seed in the greenhouse for approximately six months. Seeds were germinated in 8-cm diameter pots of soil gathered from Larimer County, CO, and soils were maintained near saturation with watering.
Extracted molecule total RNA
Extraction protocol RNeasy kit (quiagen)
Label CY3
Label protocol Genisphere Array900
 
Channel 2
Source name EXP
Organism Arabidopsis thaliana
Characteristics Centaurea maculosa root
seed source: Missoula Montana, plant ~6 months old (used same plant as was used for leaf tissue)
Biomaterial provider Seeds obtained from Callaway at the Unviersity of MT, Missoula
Growth protocol Invasive Centaurea maculosa plants from Missoula, MT were grown from seed in the greenhouse for approximately six months. Seeds were germinated in 8-cm diameter pots of soil gathered from Larimer County, CO, and soils were maintained near saturation with watering.
Extracted molecule total RNA
Extraction protocol RNeasy kit (quiagen)
Label CY5
Label protocol Genisphere Array900
 
 
Hybridization protocol Genisphere Array900
Scan protocol Axon GenePix 3.0
Description ABRO_OAR27K-508_9-9-05
Data processing Data processing VALUE is log2 (CY5/CY3; exp/ref)
 
Submission date Apr 03, 2007
Last update date Jul 24, 2008
Contact name Amanda Broz
E-mail(s) akbroz@lamar.colostate.edu
Phone 970-491-4266
Organization name Colorado State University
Department Horticulture and Lanscape Architecture
Lab Jorge Vivanco
Street address 1173 Campus Delivery
City Fort Collins
State/province CO
ZIP/Postal code 80523
Country USA
 
Platform ID GPL988
Series (1)
GSE7443 Centaurea maculosa preliminary study

Data table header descriptions
ID_REF Identifier reference, ID from the platform id
VALUE log2 (CY5/CY3; exp/ref)
CH1I_MEDIAN F532 Median: median feature pixel intensity at wavelength (532 nm)
CH1B_MEDIAN B532 Median: median feature background intensity at wavelength (532 nm)
CH2I_MEDIAN F635 Median: median feature pixel intensity at wavelength (635 nm)
CH2B_MEDIAN B635 Median: median feature background intensity at wavelength (635 nm)

Data table
ID_REF VALUE CH1I_MEDIAN CH1B_MEDIAN CH2I_MEDIAN CH2B_MEDIAN
3203163 -1.047 518 330 507 416
3203165 -5.238 694 279 426 415
3203167 -3.386 448 333 459 448
3203169 -5.597 759 275 432 422
3203171 -1.880 467 305 483 439
3203173 -2.601 556 283 477 432
3203177 -1.536 383 296 451 421
3203199 -1.295 418 261 489 425
3203203 -0.290 359 282 492 429
3203205 -1.123 419 284 516 454
3203207 -1.909 491 292 458 405
3203213 -3.473 1142 276 481 403
3203217 -1.446 493 275 489 409
3203221 -3.773 569 282 447 426
3203231 -4.902 1634 259 453 407
3203235 -3.920 1332 257 488 417
3203237 -2.862 532 263 452 415
3203263 -2.157 549 335 499 451
3203267 -3.986 572 271 433 414
3203275 -3.170 622 262 449 409

Total number of rows: 4160

Table truncated, full table size 126 Kbytes.




Supplementary file Size Download File type/resource
GSM180284.txt.gz 2.6 Mb (ftp)(http) TXT

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