|
Status |
Public on Apr 20, 2016 |
Title |
MPP_MLV |
Sample type |
SRA |
|
|
Source name |
Myeloid progenitors/precursors (MPP)
|
Organism |
Homo sapiens |
Characteristics |
cell type: CD34+-derived human myeloid progenitors/precursors tissue: Human umbilical cord blood
|
Growth protocol |
Committed erythroid and myeloid progenitors/precursors (EPP and MPP) were isolated as pools of CD34lowCD36high and CD34-CD13+ populations, after induction for five days in culture in the presence of SCF, IL3 and either Epo or G-CSF, respectively
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted and digested with MseI. 3’ LTR vector-genome junctions were amplified by linker-mediated PCR (LM-PCR), adapted to the GS-FLX Genome Sequencer (Roche/454 Life Sciences, Branford, CT) pyrosequencing platform.
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
454 GS FLX |
|
|
Data processing |
Library strategy: Retroviral scanning (MLV) Data analysis performed as previously described (Cattoglio et al. 2010b). Genome_build: Hg19
|
|
|
Submission date |
Jul 09, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Silvio Bicciato |
E-mail(s) |
silvio.bicciato@unipd.it
|
Phone |
+39-049-827-6108
|
Organization name |
University of Padova
|
Department |
Molecular Medicine
|
Street address |
via U. Bassi 59/b
|
City |
Padova |
ZIP/Postal code |
35131 |
Country |
Italy |
|
|
Platform ID |
GPL9186 |
Series (2) |
GSE70676 |
Genome-wide definition of regulatory elements in hematopoietic stem cell differentiation [Retroviral scanning (MLV-Seq)] |
GSE70677 |
Genome-wide definition of regulatory elements in hematopoietic stem cell differentiation |
|
Relations |
BioSample |
SAMN03276533 |
SRA |
SRX832052 |
SRA |
SRX832054 |
SRA |
SRX832055 |