|
| Status |
Public on Apr 20, 2016 |
| Title |
HSPC_MLV |
| Sample type |
SRA |
| |
|
| Source name |
Hematopoietic stem/progenitor cells (HSPC)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human CD34+ stem/progenitor cells
tissue: Human umbilical cord blood
|
| Growth protocol |
Committed erythroid and myeloid progenitors/precursors (EPP and MPP) were isolated as pools of CD34lowCD36high and CD34-CD13+ populations, after induction for five days in culture in the presence of SCF, IL3 and either Epo or G-CSF, respectively
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and digested with MseI. 3’ LTR vector-genome junctions were amplified by linker-mediated PCR (LM-PCR), adapted to the GS-FLX Genome Sequencer (Roche/454 Life Sciences, Branford, CT) pyrosequencing platform.
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
454 GS FLX |
| |
|
| Data processing |
Library strategy: Retroviral scanning (MLV)
Data analysis performed as previously described (Cattoglio et al. 2010b).
Genome_build: Hg19
|
| |
|
| Submission date |
Jul 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL9186 |
| Series (2) |
| GSE70676 |
Genome-wide definition of regulatory elements in hematopoietic stem cell differentiation [Retroviral scanning (MLV-Seq)] |
| GSE70677 |
Genome-wide definition of regulatory elements in hematopoietic stem cell differentiation |
|
| Relations |
| BioSample |
SAMN00113511 |
| SRA |
SRX027104 |
