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Sample GSM182106 Query DataSets for GSM182106
Status Public on Apr 17, 2007
Title Genome Input G0 phase Replicate 1
Sample type genomic
Source name Input chromatin from T98G cells
Organism Homo sapiens
Characteristics human glioblastoma cell line T98G CRL-1690
Treatment protocol Cells were incubated in serum-free medium for 3 days to induce G0 arrest
Growth protocol Human T98G cells grown till confluency in DMEM supplemeted with 10 % fetal calf serum
Extracted molecule genomic DNA
Extraction protocol Chromatin was prepared from formalin-fixated cells exactly as described in Rayman, Takahashi et al. (2002) Genes Dev 16, 933-947
Label biotin
Label protocol The chromatin immunprecipitated DNA (ChIP) or 2 ng of whole genomic DNA (Input) were amplified by ligation-mediated PCR, digested with Dnase I to give fragments of ~50 bp.
The DNA fragments were end-labelled with biotin in a TdT-catalyzed reaction.
Hybridization protocol Affymetrix fluidics protocol EukGE-WS2v4_450
Scan protocol Scanning was carried out on an Affymetrix scanner GCS3000. The scan protocol was contained in a .cif file for chip type Hs_PromPR_v02.
Description Input chromatin from G0-arrested T98G cells, replicate 1 out of 3
Data processing Affymetrix GCOS processed image files to generate .CEL files.
MAT (Model-based Analysis for Tiling Arrays) analysis algorithm used to detect regions enriched by chromatin immunoprecipitation (ChIP) on Affymetrix tiling array (ChIP-chip).
Submission date Apr 13, 2007
Last update date Mar 09, 2012
Contact name Xiaopeng Zhu
Organization name Institute of Biophysics, Chinese Academy of Sciences
Street address 15 Datun Street
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL5082
Series (1)
GSE7516 ChIP-chip analysis of human DREAM complex subunits in G0-arrested and S-phase synchronized cells

Supplementary file Size Download File type/resource
GSM182106.CEL.gz 17.3 Mb (ftp)(http) CEL
GSM182106_CEL.txt.gz 28.6 Mb (ftp)(http) TXT
Processed data are available on Series record

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