|
| Status |
Public on Jan 31, 2016 |
| Title |
SpT KO A |
| Sample type |
RNA |
| |
|
| Source name |
murine placenta
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129SV
tissue: junctional zone of placenta
developmental stage: E13.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The placenta was snap frozen in liquid nitrogen. 10 micrometer sections were cut and the junctional zone of control and mutant placenta was extracted using laser microdissection. RNA was isolated using Rneasy Micro Kit (Qiagen).Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
3 biological replicates pooled
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 15, 2015 |
| Last update date |
Jan 31, 2016 |
| Contact name |
Neha Sharma |
| E-mail(s) |
ns.nehasharma01@gmail.com
|
| Organization name |
Institute of Pathology
|
| Street address |
sigmund freud str-25
|
| City |
bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE70962 |
Analysis of Junctional zone in murine placenta upon loss of Tfap2c |
|
