|
| Status |
Public on Feb 24, 2016 |
| Title |
OVCA429 with shGRHL2 OV429_shG210_1 |
| Sample type |
RNA |
| |
|
| Source name |
Ovarian cancer cell line OVCA429
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: OVCA429
shconstruct: shGRHL2_10
|
| Treatment protocol |
Cells were infected with shRNAs (control and GRHL2-targeting shRNAs) by lentivirus, followed by puromycin selection.
|
| Growth protocol |
Cells were grown in DMEM 10% FBS, 37°C in 5% CO2, 3µg/ml puromycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen miRNEasy Mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the NuGEN Applause WT-Amp ST System and Encore Biotin Module.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials. Fragmented and biotin labeled cDNA was hybridized to Affymetrix Human Gene 2.0 ST arrays for 18 hours at 45C with rotation at 60 rpm. Arrays were then washed and stained using the FS450_0002 fluidics protocol
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
GRHL2 knockdown
|
| Data processing |
RMA normalization using R package Affy 1.42.3
HuGene-2_0-st.pgf
HuGene-2_0-st.mps
|
| |
|
| Submission date |
Jul 16, 2015 |
| Last update date |
Feb 24, 2016 |
| Contact name |
Tuan Zea Tan |
| E-mail(s) |
csittz@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute Singapore
|
| Street address |
14 Medical Drive, #11-01, Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE71017 |
Grainyhead-like 2 (GRHL2) maintains the epithelial status of ovarian cancer through miR-200-ZEB1 regulatory networks (expression) |
| GSE71019 |
Grainyhead-like 2 (GRHL2) maintains the epithelial status of ovarian cancer through miR-200-ZEB1 regulatory networks |
|
