|
| Status |
Public on Jul 21, 2015 |
| Title |
Regeneration samples at 10 days AG, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Regenerating tissues from the trunks of individual (NO. 20) at 10 days AG
|
| Organism |
Populus tomentosa |
| Characteristics |
tissue: Regeneration tissues of poplar trucks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated from these samples using LC Science Total RNA Purification Kit (LC sciences, #TRK-1001), according to the manufacturer’s instructions with a modification.
|
| Label |
biotin
|
| Label protocol |
IVT Labeling cRNA were prepared according to Affymetrix GeneChip® 3’ IVT Express Kit protocol from 3 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized at 45℃ and 60 rpm for 16 hr on GeneChip Poplar Genome Array in GeneChip® Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Data processing |
The data were analyzed with Affymetrix GeneChip Operating Software (GCOS) using default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jul 20, 2015 |
| Last update date |
Jul 21, 2015 |
| Contact name |
Fang Tang |
| E-mail(s) |
tf831121@126.com
|
| Phone |
86 010 62889359
|
| Organization name |
chinese Academy of Forestry
|
| Street address |
Xiangshan Road， Haidian district
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100091 |
| Country |
China |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE71094 |
Expression values from 6 time points during the regeneration of secondary vascular System (SVS) |
|
