|
| Status |
Public on Jun 12, 2017 |
| Title |
5 miRNA |
| Sample type |
RNA |
| |
|
| Source name |
human endometrium
|
| Organism |
Homo sapiens |
| Characteristics |
disease: RIF (recurrent implantation failure)
gender: female
age: 23
tissue: Endometrium
collect time: Window of implantation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Labeled RNAs were heat denatured and hybridized to Agilent miRNA microarray, according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
According to the Agilent miRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
After hybridization and post-hybridization washes, slides were scanned immediately in Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Description |
Gene expression without treatment
254606411001_1-3
|
| Data processing |
Feature Extraction v10.7.3.1 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Jul 24, 2015 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Fan Li juan |
| E-mail(s) |
493822506@qq.com
|
| Phone |
18811778279
|
| Organization name |
Peking University Peoples' Hospital
|
| Department |
Reproductive medical Center
|
| Lab |
Reproductive medical Center lab
|
| Street address |
11th Xizhimen south street,Xicheng district
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100044 |
| Country |
China |
| |
|
| Platform ID |
GPL18402 |
| Series (1) |
| GSE71332 |
Abberant miRNA expression profiles in recurrent implantation failure endometrium |
|
