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Sample GSM1833822 Query DataSets for GSM1833822
Status Public on Jul 18, 2017
Title TO_143_13June08
Sample type RNA
 
Source name blood cells
Organism Vulpes lagopus
Characteristics tissue: blood
Biomaterial provider The University of Washington, Seattle, WA.
Extracted molecule total RNA
Extraction protocol All blood samples destined for molecular analyses were collected in PAXgene (Qiagen) RNA tubes and stored on ice in the field until they were placed in -20°C or -80°C freezers. RNA was extracted using PaxGene Blood miRNA Kit (Qiagen) and quantity and integrity determined using an Agilent 2100 Bioanalyzer (Agilent Technologies) or a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific).
Label biotin
Label protocol For microarray hybridization, RNA was processed for hybridization to Affymetrix Canine Genome 2.0 Array through a core facility in the Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer's instructions.
 
Hybridization protocol The standard hybridization protocol was used as recommended by Affymterix.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics & Proteomics Facility Core Laboratory in the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/members/core-services.html).
Description blood cells
Data processing Affymetrix Expression Console, RMA normalization log2
 
Submission date Jul 27, 2015
Last update date Sep 05, 2017
Contact name James William MacDonald
E-mail(s) jmacdon@uw.edu
Organization name University of Washington
Department Environmental and Occupational Health Sciences
Street address 4225 Roosevelt Way NE
City Seattle
State/province WA
ZIP/Postal code 98105-6099
Country USA
 
Platform ID GPL3738
Series (1)
GSE71407 Using Domestic and Free-Ranging Arctic Canid Models for Environmental Molecular Toxicology Research

Data table header descriptions
ID_REF
VALUE RMA normalized signal log2

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.596686
AFFX-BioB-5_at 7.938108
AFFX-BioB-M_at 9.095174
AFFX-BioC-3_at 10.33254
AFFX-BioC-5_at 9.986559
AFFX-BioDn-3_at 13.22373
AFFX-BioDn-5_at 11.0842
AFFX-Cfa-actin-3_s_at 13.73278
AFFX-Cfa-actin-5_at 13.19855
AFFX-Cfa-actin-5_s_at 8.85816
AFFX-Cfa-actin-M_x_at 13.56956
AFFX-Cfa-ef1a-3_x_at 14.62646
AFFX-Cfa-ef1a-5_at 8.644217
AFFX-Cfa-ef1a-M_x_at 11.56693
AFFX-Cfa-gapdh-3_x_at 14.27782
AFFX-Cfa-gapdh-5_x_at 8.681348
AFFX-Cfa-gapdh-M_x_at 13.7411
AFFX-Cfa-r2-Bs-dap-3_at 13.20392
AFFX-Cfa-r2-Bs-dap-5_at 8.384657
AFFX-Cfa-r2-Bs-dap-M_at 13.07941

Total number of rows: 43035

Table truncated, full table size 1230 Kbytes.




Supplementary file Size Download File type/resource
GSM1833822_TO_143_13June08.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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