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Sample GSM183560 Query DataSets for GSM183560
Status Public on May 01, 2008
Title ErbB4_Doxorubicinvs.WT_4
Sample type RNA
 
Channel 1
Source name wildtype mice
Organism Mus musculus
Characteristics strain: C57Bl/6
gender: male
age: 10 months
tissue: whole heart
Biomaterial provider Cecilia Hertig, INGEBI-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires
Treatment protocol No treatment was applied.
Growth protocol Animals were kept in a SPF barrier under standardisized conditions with respect to temperature and humidity, and were housed on a 12h light/12h dark cycle in groups of 4-5 with free access to food and water. Animal housing, care, and applications of experimental procedures complied with the Guide for the Care and Use of Laboratory Animals of the Government of Berlin, Germany and are in accordance to the recommendations of the Society for Laboratory Animal Science (GV-SOLAS) and the Federation of European Laboratory Animal Science Associations (FELASA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol Reagent (Invitrogen) according to manufacturer`s instructions.
Label Cy3
Label protocol Labeling of the targets during reverse transcription was performed as described by Pat Brown (http://brownlab.stanford.edu/).
 
Channel 2
Source name ErbB4 knockout mice treated with Doxorubicin
Organism Mus musculus
Characteristics strain: C57Bl/6
gender: male
age: 10 months
tissue: whole heart
Biomaterial provider Cecilia Hertig, INGEBI-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires
Treatment protocol ErbB4 knockout mice were treated 3 times a week with 15mg/kg Doxorubicin.
Growth protocol Animals were kept in a SPF barrier under standardisized conditions with respect to temperature and humidity, and were housed on a 12h light/12h dark cycle in groups of 4-5 with free access to food and water. Animal housing, care, and applications of experimental procedures complied with the Guide for the Care and Use of Laboratory Animals of the Government of Berlin, Germany and are in accordance to the recommendations of the Society for Laboratory Animal Science (GV-SOLAS) and the Federation of European Laboratory Animal Science Associations (FELASA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol Reagent (Invitrogen) according to manufacturer`s instructions.
Label Cy5
Label protocol Labeling of the targets during reverse transcription was performed as described by Pat Brown (http://brownlab.stanford.edu/).
 
 
Hybridization protocol Targets were vacuum-centrifuged and resuspended in 50 μl DIG hybridisation buffer (Roche). Denatured targets (98°C, 2 min) were hybridised in a humidified chamber at 42°C overnight. The following day arrays were washed (2xSSC; 0,03% SDS for 10min, 2xSSC for 5min, 1xSSC for 5min, 0,2 x SSC for 2min) and dried in a centrifuge.
Scan protocol Agilent Microarray scanner (G2565BA)
Description The gene expression of the whole heart of ErbB4 knockout mice treated with doxorubicin was compared with wildtype mice.
Data processing Local background was subtracted and LOWESS normalisation was performed using Feature Extraction Software (version A 6.1.1).
 
Submission date Apr 23, 2007
Last update date Jun 05, 2007
Contact name Henning Witt
E-mail(s) witt@molgen.mpg.de
Phone ++49-30-450578727
Organization name Max Planck Institute for Molecular Genetics
Department Hans Lehrach
Lab Patricia Ruiz
Street address Ihnestrasse 65
City Berlin
ZIP/Postal code 14195
Country Germany
 
Platform ID GPL872
Series (2)
GSE7746 Gene expression profiling on mouse models of dilated cardiomyopathy
GSE17749 Gene expression profiling on mouse models of cardiomyopathies

Data table header descriptions
ID_REF
VALUE log10 ratio of the normalized intensities (CH2/CH1)
CH1_SIG_Mean mean signal intensity of the Cyanine 3 labeled target
CH1_BKD_Mean mean background signal intensity of the Cyanine 3 labeled target
CH2_SIG_Mean mean signal intensity of the Cyanine 5 labeled target
CH2_BKD_Mean mean background signal intensity of the Cyanine 5 labeled target

Data table
ID_REF VALUE CH1_SIG_Mean CH1_BKD_Mean CH2_SIG_Mean CH2_BKD_Mean
1 -0.0549373 4066.69 951.499 4560.15 268.756
2 -0.735444 1038.58 949.822 340.415 272.718
3 -0.100504 921.51 944.359 294.53 276.703
4 -0.73839 1159.34 939.83 402.252 277.416
5 -0.0755018 915.279 941.326 312.067 277.747
6 -0.756916 1145.75 932.074 395.627 275.997
7 -0.226622 894.468 919.524 316.081 272.26
8 0.148449 1542.23 925.431 1799.67 268.682
9 0.330952 874.625 916.975 473.269 273.395
12 0.63314 1085.85 911.55 2006.24 269.021
14 0.010248 1580.98 918.48 1463.75 272.886
15 0.162083 1015.5 914.699 742.657 274.574
16 0.000374137 5272.61 904.402 6982.16 277.583
17 0.00436297 1958.8 901.849 2062.42 275.452
19 -0.0833167 3351.06 907.768 3464.15 270.816
20 0.318771 906.524 909.586 589.049 268.21
21 0.0157956 9132.65 934.277 13092.7 269.62
22 0.0206047 1477.87 943.185 1276.6 263.898
23 0.0380756 1041.93 953.193 600.904 260.998
24 0.00490991 4960.73 951.669 6501.79 261.797

Total number of rows: 9653

Table truncated, full table size 435 Kbytes.




Supplementary file Size Download File type/resource
GSM183560.xml.gz 1.1 Mb (ftp)(http) XML
Processed data included within Sample table

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