|
Status |
Public on Aug 01, 2015 |
Title |
Tri6_Mutant-Replicate-2 |
Sample type |
SRA |
|
|
Source name |
Tri6_Mutant cells
|
Organism |
Fusarium graminearum |
Characteristics |
strain: Tri6_Mutant genotype/variation: Tri6_Mutant
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol (Invitrogen, USA) and InviTrap Spin Cell RNA mini kit (Invitek, Germany) were used for RNA isolation Illumina DGE Tag Profiling NlaIII Sample Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
ELAND was used for read alignment DESeq was used for data normalization and relative comparison between strains Genome_build: Fusarium graminearum strain PH-1 Supplementary_files_format_and_content: delimited text files were generated for TAG COUNT and GENE COUNT data
|
|
|
Submission date |
Jul 31, 2015 |
Last update date |
Aug 01, 2015 |
Contact name |
Rajagopal Subramaniam |
E-mail(s) |
subramaniamra@agr.gc.ca
|
Organization name |
Agriculture and Agri-Food Canada
|
Street address |
960 Carling
|
City |
Ottawa |
State/province |
Ontario |
ZIP/Postal code |
K1A0C6 |
Country |
Canada |
|
|
Platform ID |
GPL20760 |
Series (1) |
GSE71604 |
Leucine metabolism regulates TRI6 expression and affects deoxynivalenol production and virulence in Fusarium graminearum |
|
Relations |
BioSample |
SAMN03946215 |